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lamp1  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank lamp1
    Lamp1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 201 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lamp1/anti-LAMP-1/pmc13126347-320-17-19
    Average 96 stars, based on 201 article reviews
    lamp1 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: ANXA11 suppression restores muscular function in the mdx mouse model of Duchenne muscular dystrophy (DMD).
    Article Snippet: .. Cells were incubated with MyHC antibody (for differentiated C2C12) (MF20, Developmental Studies Hybridoma Bank) or mTOR (2983S, Cell Signaling Technology) and LAMP1 (1D4B, Developmental Studies Hybridoma Bank) antibody (for mTOR and lysosomes colocalization analysis) overnight at 4 °C, followed by incubation with Alexa Fluor–conjugated secondary antibodies (1:200) (EarthOx Life Sciences). .. Nuclei were stained with Hoechst (1:1000) (Coolaber).

    Article Title: Functional border-associated macrophages limit Alzheimer’s Disease progression
    Article Snippet: Sections were rehydrated 2x 5 min in PBS followed by incubation for 2hrs in staining buffer: .1% triton x-100, .05% Tween-20, 2μg/mL heparin sodium salt (H3393, Sigma-Aldrich), .01% sodium azide in 1xPBS. .. Sections were incubated ON at 4C with the following primary antibodies Goat anti-CD31 (1:150, AF3628, R& D systems), Rabbit anti-IBA1 (1:1000, 019-19741, Wako), Guinea Pig anti-NeuN (1:1000, 266 004, Synaptic systems), Rat anti-CD206 (1:500, MCA2235, Bio-Rad), Rat anti LAMP1 (1:250, 1D4B, DSHB), Rabbit anti-Laminin (1:300, Thermo Fisher, PA116730). .. The next day samples were washed 3x 10 min with staining buffer and incubated for 2hrs at RT in the following secondary antibodies Donkey anti-Goat Alexa Fluor 488 (1:200, 705-545-003 JacksonImmuno), Donkey anti-Rabbit Alexa Fluor 568 (1:500, 711-575-152, JacksonImmuno), Donkey anti-Goat Alexa Fluor 647 (1:200, 705-607-003, JacksonImmuno) Donkey anti-Rat Alexa Fluor 647 (1:500, 712-605-152, JacksonImmuno), Donkey anti-Rat Alexa Fluor 568 (1:500, 712-575-153, JacksonImmuno) Goat anti-Guinea Pig Alexa Fluor 488 (1:500, A-11073, ThermoFisher).

    Article Title: Loss of PINK1 impairs mitophagy and accelerates ovarian aging independent of Parkin.
    Article Snippet: PINK1 and Parkin are central regulators of mitophagy, a quality-control process essential for mitochondrial homeostasis and implicated in aging.. However, their specific roles in ovarian physiology remain unclear.. Here, we show that Pink1 deletion in mice leads to decreased ovarian weight, diminished ovarian reserve, and reduced oocyte quality, accompanied by increased granulosa cell apoptosis, accelerated ovarian ageing, and impaired fertility.

    Immunolabeling:

    Article Title: Use of a human immortalized microglia cell line to study recognition, phagocytosis, and intracellular survival of Cryptococcus neoformans
    Article Snippet: .. Cells were immunolabeled in 1% goat serum at 4°C overnight with an antibody against Gal-3 (rat; M3/38: sc23938, Santa Cruz Biotechnology; 1:100 concentration), LAMP1 (mouse; Developmental Studies Hybridoma Bank, H4A3; 1:500 concentration), EEA1 (rabbit; Abcam, ab2900; 1:100 concentration), or vATPase (mouse; Santa Cruz Biotechnology, D-11; 1:500 concentration). .. Coverslips were washed with PBS, and a corresponding Alexa Fluor-conjugated goat secondary antibody (Invitrogen) was diluted to 1 μg/mL in PBS with 2 μg/mL of 4′6-diamidino-2-phenylindole (DAPI; Millipore Sigma) and added to cells for 1 h at RT.

    Concentration Assay:

    Article Title: Use of a human immortalized microglia cell line to study recognition, phagocytosis, and intracellular survival of Cryptococcus neoformans
    Article Snippet: .. Cells were immunolabeled in 1% goat serum at 4°C overnight with an antibody against Gal-3 (rat; M3/38: sc23938, Santa Cruz Biotechnology; 1:100 concentration), LAMP1 (mouse; Developmental Studies Hybridoma Bank, H4A3; 1:500 concentration), EEA1 (rabbit; Abcam, ab2900; 1:100 concentration), or vATPase (mouse; Santa Cruz Biotechnology, D-11; 1:500 concentration). .. Coverslips were washed with PBS, and a corresponding Alexa Fluor-conjugated goat secondary antibody (Invitrogen) was diluted to 1 μg/mL in PBS with 2 μg/mL of 4′6-diamidino-2-phenylindole (DAPI; Millipore Sigma) and added to cells for 1 h at RT.

    Purification:

    Article Title: Neuronal overexpression of Nrf2 reduces dystrophic neurites in 5XFAD Alzheimer’s disease model mice
    Article Snippet: .. The following primary antibodies were used: Iba1 (Novus, NB100-1028, 1:300), GFAP (Abcam, ab4674, 1:2000), BACE1 (Abcam, AB108394, 1:300), NeuN (Millipore, Abn91, 1:1000), LAMP1 (Developmental Studies Hybridoma Bank, 1D4B clone, purified antibody, 1:1000), p-tau181 (Cell Signaling, #12885, 1:300), Nrf2 (Santa Cruz, sc518033 (H-6) 1:100 with antigen retrieval), GFP (Abcam Ab5450, 1:1800) Aβ42 (Invitrogen #700254, 1:1000). .. Following washes, sections were incubated for 2 hours at room temperature with 1:750 dilutions of Alexa Fluor 405, 488, 568 or 647 conjugated donkey anti-mouse, goat, chicken, rat or rabbit secondary antibodies, as appropriate, along with the following stains: 300nM DAPI, and 1:15,000 dilution of 1 mg/ml Thiazine Red (ThR) or MethoxyX04 (MeX) (HelloBio).

    other:




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    Image Search Results


    BAITs promote DC activation and antigen presentation via enhanced lysosomal processing. (A) Schematic for MHC Ⅰ/Ⅱ immunostaining. DCs were treated with antigens or BAITs (12 h), then incubated in fresh medium (with TGFβ) for 24 h before staining. (B, C) Quantification of surface MHC Ⅰ (B) and MHC Ⅱ (C) on DCs (n = 8; n.s. is P > 0.05, ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (D, E) Representative immunofluorescence images of DCs (blue: nuclei; yellow: LAMP1; green: MHC Ⅰ; magenta: MHC Ⅱ). BAIT-treated DCs show strong surface MHC and minimal intracellular antigen signal, whereas antigen-treated DCs show retained antigen (red) and weak surface MHC signal. (F–H) Flow cytometric analysis of MHC Ⅰ (F), MHC Ⅱ (G), and CD80 (H) expression on murine CD11c + DCs after exposure to antigens or BAITs. (I) Quantification of MHC Ⅰ, MHC Ⅱ, and CD80 expression in DCs by flow cytometry (n = 3; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (J) Heatmap of DC activation-related gene expression after treatment with antigens or BAITs, highlighting upregulation of maturation and antigen presentation genes and downregulation of immunosuppressive genes by BAITs (n = 3). Data are presented as mean ± SD.

    Journal: Bioactive Materials

    Article Title: Countering postoperative immune suppression with a self-assembling dendritic cell nanovaccine

    doi: 10.1016/j.bioactmat.2026.05.005

    Figure Lengend Snippet: BAITs promote DC activation and antigen presentation via enhanced lysosomal processing. (A) Schematic for MHC Ⅰ/Ⅱ immunostaining. DCs were treated with antigens or BAITs (12 h), then incubated in fresh medium (with TGFβ) for 24 h before staining. (B, C) Quantification of surface MHC Ⅰ (B) and MHC Ⅱ (C) on DCs (n = 8; n.s. is P > 0.05, ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (D, E) Representative immunofluorescence images of DCs (blue: nuclei; yellow: LAMP1; green: MHC Ⅰ; magenta: MHC Ⅱ). BAIT-treated DCs show strong surface MHC and minimal intracellular antigen signal, whereas antigen-treated DCs show retained antigen (red) and weak surface MHC signal. (F–H) Flow cytometric analysis of MHC Ⅰ (F), MHC Ⅱ (G), and CD80 (H) expression on murine CD11c + DCs after exposure to antigens or BAITs. (I) Quantification of MHC Ⅰ, MHC Ⅱ, and CD80 expression in DCs by flow cytometry (n = 3; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (J) Heatmap of DC activation-related gene expression after treatment with antigens or BAITs, highlighting upregulation of maturation and antigen presentation genes and downregulation of immunosuppressive genes by BAITs (n = 3). Data are presented as mean ± SD.

    Article Snippet: After treatment, the cells were washed twice with PBS and fixed in 4% paraformaldehyde for 20 min. For intracellular protein staining, fixed cells were permeabilized with 0.1% Triton X-100 at room temperature for 10 min. Next, the cells were blocked with 2% BSA and incubated with rat CoraLite Plus 488 anti-mouse LAMP1 antibody (1:200) and rabbit anti-mouse pSAP (1:200) antibody overnight at 4 °C.

    Techniques: Activation Assay, Immunopeptidomics, Immunostaining, Incubation, Staining, Immunofluorescence, Expressing, Flow Cytometry, Gene Expression